Zebrafish tumor xenograft models

Tumor growth/regression and metastasis in zebrafish

We are unique in offering zebrafish tumor xenograft models based on implanting human or mouse tumor cells into zebrafish embryos. Small molecule drugs can be added to the water or larger therapeutic agents or cells can be co-injected with the tumor cells. These models can be combined with radiation therapy, immune-modulatory therapy, genetic interference or editing, tumor stroma analyses, molecular analyses, ADME analysis and many other services depending on your needs.

Tumor regression


Tumor growth/regression and metastasis rates are determined over three days. IC50 values and therapeutic windows are determined comparing anti-tumor efficacy with toxicity in the same animal.

Metastatis


Metastatic potential and early both local and distal dissemination (through tumor vessels) are quantified three days after tumor implantation.

Bibiography


  • Savio M, Ferraro D, Maccario C, Vaccarone R, Jensen LD, Corana F, Mannucci B, Bianchi L, Cao Y and Stivala LA (2015), “Resveratrol analogue 4,4’-dihydroxy-trans-stilbene potently inhibits cancer invasion and metastasis”, Sci. Rep., Epub

  • Ochoa-Alvarez JA, Krishnan H, Pastorino GJ, Nevel E, Kephart D, Shen Y, Fatahzadeh M, Baredes S, Kalyousef E, Honma M, Adelson ME, Kaneko MK, Kato Y, Yin K, Shienbaum AJ, Jensen LD and Goldberg GS (2015), “Antibody and lectin can target podoplanin to inhibit oral squamous carcinoma cell migration and viability”, Oncotarget, 6(11), pp. 9045-60.

  • Chen X, Wang J, Cao Z, Jensen L, Yang H, Liu Y and Cao Y (2015), “Invasiveness and metastasis of retinoblastoma in an orthotopic zebrafish tumor model”, Scientific Reports, 5, 10351.

  • Svensson S, Abrahamsson A, Olsson A-K, Jensen LD, Cao Y and Dabrosin C (2015), "CCL2 and CCL5 are novel therapeutic targets for estrogen dependent breast cancer" , Clin Cancer Res, 21(16) pp. 3794-805.

  • Fernández-Barral A, Orgaz JL, Baquero J, Ali Z, Moreno A, Tiana M, Gómez V, Riveiro-Falkenbach E, Cañadas C, Zazov S, Bertolotto C, Davidson I, Rodríguez-Peralto JL, Palmero I, Rojo F, Jensen LD, del Peso L and Jiménez B (2014) ”Regulatory and functional connection of microphthalmia-associated transcription factor and anti-metastatic pigment epithelium derived factor in melanoma”, Neoplasia, 16(6), pp. 529-42.

  • Gomez-Maldonade L, Tiana, M, Roche O, Prado-Cabrero A, Jensen LD, Fernandex-Barral A, Guijarro-Munoz I, Favaro E, Moreno-Bueno G, Sanz L, Aragones J, Harris A, Volpert O, Jimenez B and del Peso L, (2014) “EFNA3 long non-coding RNAs induced by hypoxia promote metastatic dissemination”, Oncogene, 34(20), pp. 2609-20.

  • Rouhi P, Jensen LD, Cao Z, Länne T, Wahlberg E, Steffensen JF and Cao Y, (2010), “Hypoxiainduced metastasis model in embryonic zebrafish”, Nat. Protocols, 5(12), pp. 1911-8

  • Lee SLC, Rouhi P, Dahl Jensen L, Zhang J, Ji H, Hauptmann G, Cao Y, (2009) “Hypoxia-induced pathological angiogenesis mediates tumor cell dissemination, invation and metastasis in a zebrafish tumor model”, Proc. Natl. Acad. Sci. U S A, 106(46), pp. 19485-90

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